Journal: Genes

Article Title: BAG3 Alleviates Atherosclerosis by Inhibiting Endothelial-to-Mesenchymal Transition via Autophagy Activation

doi: 10.3390/genes13081338

Figure Lengend Snippet: BAG3 blocks ox-LDL-induced EndMT through formation of CASA complex with HSP70 and HSPB8 in HUVECs. ( A ) Co-IP analysis for the interaction of BAG3 with HSP70 or HSPB8. HUVEC extracts were immunoprecipitated with anti-BAG3 antibody and probed with anti-HSP70 or anti-HSPB8 antibody. ( n = 3). ( B – H ) BAG3, HSP70, HSPB8, CD31, and α-SMA protein expressions and the LC3-II/LC3-I ratio were analyzed using Western blotting. Fold changes are shown ( n = 5). ( I ) Morphological changes post the indicated treatments were observed under a microscope (scale bar indicates 100 µm). ( J , K ) Transwell cell invasion assay was performed to analyze the migration ability of cells (scale bar indicates 100 µm). ( n = 3). ( L ) Representative images of double-fluorescent staining with α-SMA (green) and CD31 (red). The nuclei were stained blue with DAPI (scale bar indicates 50 µm). The data are presented as the mean ± S.E.M. ns p > 0.05, * p < 0.05, ** p < 0.01.

Article Snippet: To detect the interactions between HSP70 and HSPB8 and BAG3 protein, the membranes were incubated with antibodies against HSP70 (66183-1-Ig, ProteinTech Group, Inc., Chicago, IL, USA) and HSPB8 (15287-1-AP, ProteinTech Group, Inc., Chicago, IL, USA).

Techniques: Co-Immunoprecipitation Assay, Immunoprecipitation, Western Blot, Microscopy, Invasion Assay, Migration, Staining

Journal: International Journal of Biological Sciences

Article Title: Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice

doi: 10.7150/ijbs.78489

Figure Lengend Snippet: Key Resource Table

Article Snippet: Rabbit anti-TRAPPC9 , Proteintech , Cat #:16014-1-AP; RRID: AB_2256482.

Techniques: Sequencing, Reverse Transcription, SYBR Green Assay, Recombinant, Software